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dedicated complete medium  (Procell Inc)

 
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    Procell Inc dedicated complete medium
    Dedicated Complete Medium, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dedicated complete medium/product/Procell Inc
    Average 86 stars, based on 1 article reviews
    dedicated complete medium - by Bioz Stars, 2026-05
    86/100 stars

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    Localization and migration of <t>transplanted</t> <t>CXCR4-MSCs</t> in the lungs. Lung tissue of ALI rats was harvested for immunocytochemistry with anti-CXCR4 and anti-Ki67 antibodies 2 weeks post <t>MSC</t> injection. A, GFP+ MSCs (green) were observed in the lung tissue in both cases, and the majority of GFP+ MSCs were Ki67-positive (red), corresponding to the proliferation potential of the cells. B, CXCR4 was strongly expressed (red) only in the lung of rats treated with CXCR4-MSCs. The white arrows highlight cells that are double-positive to CXCR4 and GFP. Nuclei were stained with DAPI (blue). Scale bar: 100 in A, 50 μm in B. C, quantitative analysis of percentages of Ki67+/GFP+/DAPI+ cells in the total number of GFP+/DAPI+ cells in lungs of ALI rats. D, quantitative analysis of the number of GFP+ MSCs (cells/mm2) in lungs of ALI rats. Symbols represent the mean ± S.D.; n = 5 rats per group, 5 sections per rat were selected, and 10 fields were photographed in each section. Cells were counted by Image J software. Comparison between groups of CXCR4-MSCs and GFP-MSCs. **, p < 0.01.
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    Localization and migration of transplanted CXCR4-MSCs in the lungs. Lung tissue of ALI rats was harvested for immunocytochemistry with anti-CXCR4 and anti-Ki67 antibodies 2 weeks post MSC injection. A, GFP+ MSCs (green) were observed in the lung tissue in both cases, and the majority of GFP+ MSCs were Ki67-positive (red), corresponding to the proliferation potential of the cells. B, CXCR4 was strongly expressed (red) only in the lung of rats treated with CXCR4-MSCs. The white arrows highlight cells that are double-positive to CXCR4 and GFP. Nuclei were stained with DAPI (blue). Scale bar: 100 in A, 50 μm in B. C, quantitative analysis of percentages of Ki67+/GFP+/DAPI+ cells in the total number of GFP+/DAPI+ cells in lungs of ALI rats. D, quantitative analysis of the number of GFP+ MSCs (cells/mm2) in lungs of ALI rats. Symbols represent the mean ± S.D.; n = 5 rats per group, 5 sections per rat were selected, and 10 fields were photographed in each section. Cells were counted by Image J software. Comparison between groups of CXCR4-MSCs and GFP-MSCs. **, p < 0.01.

    Journal: The Journal of Biological Chemistry

    Article Title: CXCR4 Receptor Overexpression in Mesenchymal Stem Cells Facilitates Treatment of Acute Lung Injury in Rats *

    doi: 10.1074/jbc.M114.605063

    Figure Lengend Snippet: Localization and migration of transplanted CXCR4-MSCs in the lungs. Lung tissue of ALI rats was harvested for immunocytochemistry with anti-CXCR4 and anti-Ki67 antibodies 2 weeks post MSC injection. A, GFP+ MSCs (green) were observed in the lung tissue in both cases, and the majority of GFP+ MSCs were Ki67-positive (red), corresponding to the proliferation potential of the cells. B, CXCR4 was strongly expressed (red) only in the lung of rats treated with CXCR4-MSCs. The white arrows highlight cells that are double-positive to CXCR4 and GFP. Nuclei were stained with DAPI (blue). Scale bar: 100 in A, 50 μm in B. C, quantitative analysis of percentages of Ki67+/GFP+/DAPI+ cells in the total number of GFP+/DAPI+ cells in lungs of ALI rats. D, quantitative analysis of the number of GFP+ MSCs (cells/mm2) in lungs of ALI rats. Symbols represent the mean ± S.D.; n = 5 rats per group, 5 sections per rat were selected, and 10 fields were photographed in each section. Cells were counted by Image J software. Comparison between groups of CXCR4-MSCs and GFP-MSCs. **, p < 0.01.

    Article Snippet: Rat MSCs were cultured in SD rat bone marrow MSC dedicated complete medium (Cyagen Biosciences; Guangzhou, China).

    Techniques: Migration, Immunocytochemistry, Injection, Staining, Software